Thinking Like a Biotechnician

In order produce recombination DNA, you must find the restriction enzyme that matches up with both the human DNA sequence and the plasmid of bacteria DNA. Restriction enzymes are enzymes that cut the DNA sequence to attach the plasmid to the DNA. The restriction enzyme sequences Bam HI, Hpa II, and Xma appeared on our DNA sequence twice and our plamid once, making them all possible to use, however, we used Xma I because those sequences were located closest to the insulin gene. I would use the antibiotic tetracycine in our petri dish because our plasmid provided resistance to tetracycine. If our plamid actually resisted tetracycine than when placed in a dish of that antibiotic, it would not be affected. If I used an enzyme that cut the plasmid in two places then a section of the plasmid would get cut out, and it would break apart This process is important in everyday life because as a society we use it to easily double and triple our supply of products that are used in medicine, foods, and plants. By altering the DNA sequence we can create new variations of living organisms that benefit our needs. For example, some plants are genetically engineered to protect themselves against pests and be resistant to cold temperatures, strengthening our world-wide food supply. Another example of how is process is used is cloning, an ethical issue that faces much controversy in our modern-day world.

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