pGLO Lab

1.

Plate	Number of Colonies	Color of colonies under room light	Color of colonies under   UV light
- pGLO LB	carpet	yellowish-tan	Greenish-blue
- pGLO LB/amp	none	-	-
+ pGLO LB/amp	164	yellowish-tan	Greenish-blue
+ pGLO LB/amp/ara	59	yellowish-tan	Greenish-blue

2.  We added arabinose to our e-coli, giving them the ability of glow green under a UV light. The other trait we transformed our bacteria to have is ampicillin resistance.

3. In the 100 uL of bacteria we spread across each plate, there were only one or two colonies. The agar provided nutrients for that colony, causing the bacteria to spread, leaving us with at least 50 more in the positive pGLO petri dishes. 

4. The role arabinose played was activating the gene in the bacteria to glow. It released the active repressor, allowing the RNA polymerase to read the gene, causing the cell to be fluorescent.

5.  

• Fusion tagging: used to tag living things to monitor the positioning of proteins that don't glow 
• Transcription Reporter: placing it under the promoter of interest to effectively monitor gene expression
• Optogenetics: use of light to detect, measure, and control molecular signal in a cell

6. Genetic engineering is used in agriculture to create genetically-modified crops, or genetically-modified organisms. Scientists combine the DNA of one or more organisms for some specific genetic feature. For example, plants are often genetically modified to kill off pests and survive in different climates.